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SureLight® B-Phycoerythrin ( B-PE), 1mg

$27.00

Product Description

D4-2212-1mg

Please contact us for bulk pricing.

Applications for B-Phycoerythrin

Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore. It is compatible with commonly available lasers and gives exceptional results in flow cytometry, Luminex® and immunofluorescent staining. B-PE is also less “sticky” than common synthetic fluorophores and therefore gives less background interference.

Structural Characteristics

B-phycoerythrin is produced by red algae such as Rhodella sp.. The protein is made up of at least three different subunits and varies according to the species of algae that produces it. The subunit structure of the most common B-PE is (αβ)6γ. The α subunit has two phycoerythrobilins (PEB), the β subunit has 3 PEBs and the γ subunit has 2 PEB and 2 phycourobilins (PUB). The molecular weight of B-PE is 240,000 Daltons.

Excitation and emission profiles for SureLight® B-PE. (Emission scan excitation wavelength at 540 nm.) Compatible with:

  • 532 nm - Diode-Pumped Solid State (DPSS) Laser
  • 543 nm - He/Ne Laser
 

Spectral Characteristics

Absorption maximum 545 nm
Additional Absorption peak 563.5
Emission maximum 572 nm
Extinction Coefficient (ε) 2.41 x 106 M-1cm-1
Quantum Yield (QY) 0.98
Brightness (ε x QY) 2.36 x 106 M-1cm-1

Specifications

A545/A280 ≥ 5.5
A620/A545 < 0.01
 
>95% single peak by HPLC

Lot-specific Technical Data Sheets

 

General Information Sheet

MSDS

Technical Data Sheet

 


 
 
 

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Quantity:

SKU:
D4-000